Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation
J. Clin. Invest. Alexander Zarbock, et al. 116:3211 doi:10.1172/JCI29499 [Go to this article.]

Figure 2
Acid-induced ALI causes platelet-neutrophil interactions. (A) Flow cytometry analysis of platelet-neutrophil aggregates after initiation of HCl-induced ALI. CD45, Gr-1, and 7/4 mAbs were used to identify PMNs (data not shown). Neutrophil-platelet aggregates were identified as neutrophils that were also positive for the platelet-specific marker CD41. (B) Thirty minutes after initiation of HCl-induced ALI, platelet-neutrophil interaction in the blood increased significantly. P-selectin Abs almost completely prevented the formation of platelet-neutrophil aggregates (n = 6–9 mice per group). (C) Platelet depletion by Abs prior to initiation of acid-induced ALI reduced the amount of platelet-neutrophil interactions in the blood. (D) Platelet-neutrophil interactions 30 minutes after initiation of acid-induced ALI in pulmonary microvasculature visualized by electron microscopy. Platelets (arrows) attached directly to the endothelium and a neutrophil. Scale bar: 1 μm. Original magnification, ×8000. ^#P < 0.05. P-sel, P-selectin.