Ablation of Cbl-b provides protection against transplanted and spontaneous tumors
J. Clin. Invest. Jeffrey Y. Chiang, et al. 117:1029 doi:10.1172/JCI29472 [Go to this article.]

Figure 2
Eradication of inoculated tumors in Cblb^–/– mice. (A) Growth rates of inoculated E.G7 tumors and survival of tumor-bearing mice. After 10⁶ E.G7 cells were inoculated into the flanks of WT or Cblb^–/– mice by s.c. injection, tumor growth was documented as total volume of tumor size. Left and middle: growth rates of E.G7 tumors in 1 representative experiment of 5 or more independent experiments. Each curve represents 1 mouse. Right: percentages of surviving mice (WT, n = 13; Cblb^–/–, n = 29) during the course of tumor growth. When the tumor volume reached approximately 5,000 mm³, the mice were euthanized and recorded as dead. (B) Growth rates of EL4 tumors in Cblb^–/– and WT mice. EL4 cells (5 × 10⁴ cells/mouse) were injected s.c. into the flanks of 5 WT and 5 Cblb^–/– mice, and tumor growth was monitored over time. WT mice included both Cblb^+/+ (n = 16) and Cblb^+/– (n = 4) mice. The data presented are representative of 4 independent experiments. (C) Rejection of EL4 tumors by Cblb^–/–CD28^–/– mice. EL4 cells (5 × 10⁴ cells/mouse) were injected, and tumor growth was monitored as described in B. The number of mice with tumor growths is indicated at the top of each column.