Antigen-specific immune responses to influenza vaccine in utero
J. Clin. Invest. Deepa Rastogi, et al. 117:1637 doi:10.1172/JCI29466 [Go to this article.]

Figure 7
Maternal and neonatal influenza-specific CD4⁺ T cells are CD45RO positive. Representative maternal PBMCs and CBMCs were stained with DR4/HA tetramers and anti-CD45RO antibody versus isotype control (data for the latter not shown). CD4⁺/DR4/HA⁺ T cell population was gated to quantify the CD45RO⁺ T cells. Background staining of postpartum maternal HLA DRB1*0401^– PBMCs was 0.008%. Bottom panel indicates that maternal PBMCs and newborn CBMCs that stained with DR4/HA tetramer also expressed CD45RO (50% and 66.7% DR4/HA⁺ cells, respectively). Panels show percentage of tetramer-staining–positive cells from a representative subject.