Figure 5
Representative comparison of tetramer staining in DRB1*0401
+ and DRB1*0401
– cord blood.
(A and B) DR4 HLA typing by flow cytometry. (C) DRB1*0401+-HA T cell clone (HA136). (D) DRB1*0401+ inherited from father; staining performed with DR4/HA tetramer. (E) DRB1*0401+ inherited from father; staining performed with DR4/GAD tetramer. (F) DRB1*0401– newborn; staining performed with DR4/HA tetramer. (G) DRB1*0401– newborn; staining performed with DR4/GAD tetramer. Panels show percentage of tetramer-staining–positive cells from a representative subject.
