Vitamin D receptor in chondrocytes promotes osteoclastogenesis and regulates FGF23 production in osteoblasts
J. Clin. Invest. Ritsuko Masuyama, et al. 116:3150 doi:10.1172/JCI29463 [Go to this article.]

Figure 4
Vascular invasion and osteoclast formation are impaired in E15. Cre⁺VDR^fl/fl mice. (A–D, F–I) Sets of adjacent tibial sections were immunostained for CD31 (A–D) or TRAP (F–I) to visualize endothelial cells or osteoclasts in E15.5 Cre^–VDR^fl/fl (A, B, F, and G) and Cre⁺VDR^fl/fl (C, D, H, and I) mice, respectively. Scale bar: 200 μm. (E) Invasion of blood vessels from the perichondrium into the cartilage core was measured and expressed relative to the cartilage width in Cre⁺VDR^fl/fl and Cre^–VDR^fl/fl tibiae. (J) Quantification of the number of osteoclasts (Oc.N) in the primary ossification center. *P < 0.05 versus Cre^–VDR^fl/fl.