Vitamin D receptor in chondrocytes promotes osteoclastogenesis and regulates FGF23 production in osteoblasts
J. Clin. Invest. Ritsuko Masuyama, et al. 116:3150 doi:10.1172/JCI29463 [Go to this article.]

Figure 3
Chondrocyte-specific VDR inactivation results in increased metaphyseal bone volume in neonatal and 15-day-old mice. (A) Quantification of BV/TV in the proximal tibiae metaphysis on von Kossa–stained sections shows a significant increase in BV/TV in Cre⁺VDR^fl/fl mice compared with Cre^–VDR^fl/fl mice. (B and C) von Kossa staining on tibiae sections of 15-day-old Cre^–VDR^fl/fl (B) and Cre⁺VDR^fl/fl mice (C). Scale bar: 400 μm. (D) Osteocalcin gene expression in femora (total bone or dissected diaphysis) of 15-day-old Cre^–VDR^fl/fl and Cre⁺VDR^fl/fl mice was assessed by qRT-PCR analysis and calculated as a ratio to the HPRT mRNA copies. Cre^–VDR^fl/fl value was set at 100%. *P < 0.05; **P < 0.01 versus Cre^–VDR^fl/fl.