S-Nitrosothiols signal hypoxia-mimetic vascular pathology
J. Clin. Invest. Lisa A. Palmer, et al. 117:2592 doi:10.1172/JCI29444 [
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Figure 2Three weeks of NAC treatment or hypoxia increases the whole-lung expression of certain genes associated with the development of PAH in mice. The expression of fibronectin (
A), HIMF (
B), eNOS (
C and
D), VEGF-A (
E), and endothelin (
F) in whole-lung homogenates from NAC-treated mice was examined by immunoblot. Fold increase in density relative to MAPK (equal loading control) was determined for each condition. The increases in fibronectin, HIMF, and eNOS (
n = 3–5 each) were significant. (
G) Three weeks of NAC treatment also increased whole-lung mRNA, assayed relative to 18S RNA by RT-PCR, for
HIMF and
VEGF-A but not
fibronectin or
Glut-1 (
n = 3 each). Time course analysis of NAC-treated mice (
D) revealed that the increase in whole-lung
eNOS mRNA (filled squares, left axis) preceded the increase in eNOS protein expression (open circles, right axis) but decreased by 3 weeks. *
P < 0.05;
#P < 0.01.
