Neuronal SH2B1 is essential for controlling energy and glucose homeostasis
J. Clin. Invest. Decheng Ren, et al. 117:397 doi:10.1172/JCI29417 [
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Figure 4Neuron-specific restoration of SH2B1 reverses leptin resistance in SH2B1
KO mice.
(
A) Plasma leptin levels in fasted (10 weeks old) and fed ad libitum (13 weeks old) mice. (
B) Male mice (9 weeks old) were housed individually and injected intraperitoneally with leptin (2 mg/kg body weight) or PBS (control) twice a day (6:00 pm and 12:00 am). Body weight was monitored both before and after the injection. Changes in body weight were calculated as a percentage of the initial values prior to the injection. (
C) Female (right panel: 6 weeks old) and male (middle panel: 12 weeks old; right panel: 9 weeks old) mice were fasted for 24 hours and injected intraperitoneally with leptin (1 mg/kg of body weight) or PBS as control. Hypothalamic extracts were prepared 45 minutes after injection and immunoblotted with α–p-STAT3 or α-STAT3. Each lane represents a combination of 2 hypothalami. (
D) Hypothalamic RNA was prepared from males (22 weeks old, fasted overnight). NPY, AgRP, and POMC mRNA levels were measured using quantitative real-time PCR and normalized to the expression of β-actin. *
P < 0.05.
