Neuronal SH2B1 is essential for controlling energy and glucose homeostasis
J. Clin. Invest. 117:2 doi:10.1172/JCI29417 [Go to this article.]

Figure 2
Neuronal and adipose SH2B1 have opposite effects on adiposity.

(A) Weight of epididymal (Epi) and inguinal fat depots (Ing) from SH2B1^KO, SH2B1^TgKO-437, and WT males at age 23–24 weeks. (B) Whole body fat content in SH2B1^KO, SH2B1^TgKO-437, and WT mice. (C) Representative H&E staining of epididymal fat depots from SH2B1^KO, SH2B1^TgKO-437, and WT males at age 23 weeks (upper panels) or from SH2B1^TgKO-437 and WT males at age 10 weeks (lower panels). (D) 3T3-L1 preadipocytes were differentiated into adipocytes for 0, 3, 6, or 10 days. Cell extracts were immunoprecipitated with α-SH2B1 and immunoblotted with α-SH2B1 (upper panel). Cell extracts were also immunoblotted with anti–β-actin antibodies (lower panel). (E) WT and SH2B1^KO MEF primary cultures were subjected to adipocyte differentiation for 10 days. Differentiated cells were stained with oil red O. *P < 0.05.