Neuronal SH2B1 is essential for controlling energy and glucose homeostasis
J. Clin. Invest. Decheng Ren, et al. 117:397 doi:10.1172/JCI29417 [Go to this article.]

Figure 1
Neuron-specific restoration of SH2B1β rescues obesity and hyperlipidemia in SH2B1^KO mice. (A) Tissue extracts (2 mg protein in hypothalamic and 3 mg protein in other tissue extracts) were prepared from WT mice, immunoprecipitated with α-SH2B1, and immunoblotted with α-SH2B1. Lanes 1–8 represent tissue extracts from spleen, pancreas, heart, hypothalamus, muscle, liver, white adipose tissue, and brain, respectively. (B) Schematic representation of the Myc-tagged SH2B1β transgene. (C) Brain extracts were prepared from an SH2B1^Tg mouse and a WT littermate, immunoprecipitated with α-SH2B1, and immunoblotted with α-Myc. (D) Brain extracts were prepared from WT, SH2B1^KO, and SH2B1^TgKO-437 mice and immunoblotted with α-SH2B1. Each lane represents a sample from 1 mouse. (E) Tissue extracts were prepared from the hypothalamus (Hypo), brain, skeletal muscle, liver, pancreas (Pan), white adipose tissue (WAT), brown adipose tissue (BAT), heart, and lung from an SH2B1^TgKO-437 female (16 weeks old); immunoprecipitated with α-SH2B1; and immunoblotted with α-Myc. (F) Growth curves for WT, SH2B1^Tg, SH2B1^KO, and SH2B1^TgKO-437 mice. Number in parentheses indicates the number of mice per group. (G) Levels of plasma FFAs and TGs in males (17 weeks old) fasted overnight. (H) Liver weight and TG levels in mice (21–22 weeks old) fasted overnight. *P < 0.05.