The type III TGF-β receptor suppresses breast cancer progression
J. Clin. Invest. Mei Dong, et al. 117:206 doi:10.1172/JCI29293 [
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Figure 3Frequent LOH of the
TGFBR3 gene locus in human breast cancers correlates with loss of TβRIII mRNA expression.
LOH analysis was performed on DNA extracted from 26 human breast cancer specimens and matching normal lymphocytes. (
A) Representative results showing allelic loss in tumors 1, 2, and 6 (denoted by asterisks) when PCR products were separated on a MetaPhor agarose gel. Microsatellite markers D1S1588 and D1S188 are described in Methods. (
B) LOH was confirmed using an ABI sequencer and quantified using GeneScan software. A representative sample with LOH is shown. (
C) Quantitative real-time PCR analysis of TβRIII mRNA levels in breast cancer specimens with (red bars) and without (black bars) LOH. (
D) Quantitative real-time PCR analysis of mRNA levels of TβRI, TβRII, and TβRIII in MDA-MB231 cells in response to TGF-β1 (100 pM) stimulation for the indicated times.
