Alterations in CD46-mediated Tr1 regulatory T cells in patients with multiple sclerosis
J. Clin. Invest. Anne L. Astier, et al. 116:3252 doi:10.1172/JCI29251 [Go to this article.]

Figure 3
Defect in IL-10 production is independent of the strength of T cell stimulation. (A) A group of patients with MS was either stimulated with 2 μg/ml or 10 μg/ml of anti-CD3 and anti-CD46 antibodies, and cytokine production was determined by ELISA. Although increasing CD46 cross-linking augmented IL-10 production by T cells from both healthy controls and patients with MS, there was still a significant difference between these 2 groups, with lower IL-10 secretion in patients with MS. Average IL-10 production (pg/ml): controls = 1,539, MS = 508 at 1 μg/ml; controls = 2,684, MS = 818 at 10 μg/ml. Average IFN-γ secretion: controls = 5,021, MS = 2,656 at 1 μg/ml; controls = 6,781, MS = 2,466 at 10 μg/ml. (B) T cells were stimulated at either 2 μg/ml or 10 μg/ml of anti-CD3 and anti-CD46 antibodies in the presence of increasing concentrations of IL-2, and proliferation as well as IL-10 secretion were measured. The levels of cytokine secreted were normalized to the proliferation by calculating the ratio of proliferation to IL-10 production.