JCI - ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury

ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury

Kensuke Noma, Yoshiyuki Rikitake, Naotsugu Oyama, Guijun Yan, Pilar Alcaide, Ping-Yen Liu, Hongwei Wang, Daniela Ahl, Naoki Sawada, Ryuji Okamoto, Yukio Hiroi, Koichi Shimizu, Francis W. Luscinskas, Jianxin Sun, James K. Liao

Published in Volume 118, Issue 5

J Clin Invest. 2008; 118(5):1632–1644 doi:10.1172/JCI29226

Abstract | Full text | PDF | Supplemental material

Options: View larger image (or click on image)

Figure 9

BM-derived cells contribute to leukocyte recruitment and cell proliferation in Rock1^+/– to WT BMT mice.

(A) Representative histological sections from carotid arteries in WT and Rock1^+/– BMT mice stained with CD45 Ab for leukocytes. Arrowheads indicate CD45-positive cells. Scale bar: 50 μm (left panel). Quantitative analysis of the number of CD45-positive leukocytes adherent to the unligated (n = 3–4) and ligated vessel wall at 3 days after ligation (n = 8–12). (B) Representative histological sections from carotid arteries in WT and Rock1^+/– BMT mice stained with MOMA-2 Ab for macrophages. Arrowheads indicate MOMA-2–positive cells. Scale bar: 50 μm (left panel). Quantitative analysis of the number of MOMA-2–positive macrophages infiltrated into the unligated (n = 3–4) and ligated vessel wall at days 3 (n = 7–8). (C) Representative histological sections from carotid arteries in WT and Rock1^+/– BMT mice stained for PCNA at 14 days after ligation. Arrowheads indicate PCNA-positive cells. Scale bar: 50 μm (left panel). Quantitative analysis of the ratio of PCNA-positive cells to total cell number in the intima and the media (n = 8–9). *P < 0.05 versus unligated mice; ^†P < 0.01, ^ΧP < 0.05 versus Rock1^+/– to WT mice.