ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
J. Clin. Invest. Kensuke Noma, et al. 118:1632 doi:10.1172/JCI29226 [
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Figure 7Decreased cell proliferation in the neointima of
Rock1+/– mice.
(
A) Representative histological sections from carotid arteries in WT and
Rock1+/– mice stained for PCNA at 14 days after ligation. Arrowheads indicate PCNA-positive cells. Scale bars: 50 μm (left panels). Quantitative analysis of the ratio of PCNA-positive cells to total cell number in the intima and the media (
n = 10–16) (right panel). *
P < 0.01 versus WT mice. (
B) Cell proliferation in response to serum of VSMCs from WT and
Rock1+/– mice. Experiments were performed 6 times in triplicates. (
C) DNA synthesis in response to PDGF of VSMCs from WT and
Rock1+/– mice (
n = 12). *
P < 0.01 versus without PDGF (control). (
D) Cell migration in response to PDGF of VSMCs from WT and
Rock1+/– mice (
n = 8–9). *
P < 0.01 versus without PDGF (control);
†P < 0.01 versus control;
ΧP < 0.01 versus WT. (
E) Representative western blot analysis of ROCK and ERK activities in VSMCs of WT and
Rock1+/– mice. VSMCs were stimulated with 10 ng/ml of PDGF for the indicated time periods.
