ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
J. Clin. Invest. Kensuke Noma, et al. 118:1632 doi:10.1172/JCI29226 [Go to this article.]

Figure 6
Decreased expression of PDGF-B, degradation of IκB-α, and activation of NF-κB in macrophages from Rock1^+/– mice. (A) Representative western blot analysis of IκB-α expression in peritoneal macrophages from WT, Rock1^+/–, and Rock2^+/– mice. Actin was used as an internal control. (B) Representative electrophoretic mobility shift assay of NF-κB in WT, Rock1^+/–, and Rock2^+/– macrophages (left panel). Specificity of NF-κB binding activity was analyzed by the addition of unlabeled probe, by the pretreatment with excess unlabeled probe (cold) or mutant probe (mutant cold), and by anti-p65 Ab (p65 Ab) supershift gel assay (right panel). NF-κB binding band (NF-κB), nonspecific binding band (NS), free probe (free), and the raised bands supershifted by Ab (supershift) are indicated on the right. (C) Expression of Pdgfa and -b were analyzed by quantitative real-time PCR. Total RNA was extracted from thioglycollate-induced peritoneal macrophages in WT, Rock1^+/–, and Rock2^+/– mice (n = 6–8). *P < 0.05 versus WT and Rock2^+/– mice.