ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
J. Clin. Invest. Kensuke Noma, et al. 118:1632 doi:10.1172/JCI29226 [
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Figure 4Decreased recruitment and adhesion of leukocytes in
Rock1+/– mice.
Neutrophil and macrophage recruitment was induced by i.p. injection of thioglycollate. (
A) Neutrophil recruitment 4 hours after i.p. injection of PBS (control) or 3% thioglycollate into WT,
Rock1+/–, and
Rock2+/– mice (
n = 10, 5, and 5, respectively). (
B) Macrophage recruitment 4 days after i.p. injection of PBS (control) or 3% thioglycollate into WT,
Rock1+/–, and
Rock2+/– mice (
n = 9, 8, and 6, respectively). Confluent monolayers of ECs isolated from WT and
Rock1+/– mice were incubated with PBS (control,
n = 4–6 in each group) or 10 units/ml of thrombin for 10 minutes (
n = 9–10 in each group). They were then placed under laminar flow at 0.5 (
C) or 1.0 dynes/cm
2 (
D). U937 monocytes, stably expressed human L selectin (U937-LAM), were perfused over the EC monolayers, and adherent cells were quantified after 3 minutes. *
P < 0.01 versus each control group;
†P < 0.05 versus each control group;
‡P < 0.01 versus
Rock1+/– mice with thioglycollate or
Rock1+/– ECs with thrombin;
ΧP < 0.05 versus
Rock1+/– mice with thioglycollate or
Rock1+/– ECs with thrombin.
