ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
J. Clin. Invest. Kensuke Noma, et al. 118:1632 doi:10.1172/JCI29226 [
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Figure 3Decreased leukocyte recruitment to ligated vessels of
Rock1+/– mice.
(
A) Representative histological sections from carotid arteries in WT and
Rock1+/– mice stained with CD45 Ab for leukocytes. Arrowheads indicate CD45-positive cells. Scale bar: 50 μm (left panel). Quantitative analysis of the number of CD45-positive leukocytes adherent to the unligated (
n = 3–4 in each group) and ligated vessel walls (
n = 14 in each group) at 3 days after ligation (right panel). (
B) Representative histological sections from carotid arteries in WT and
Rock1+/– mice stained with MOMA-2 Ab for macrophage. Arrowheads indicate MOMA-2–positive cells. Scale bar: 50 μm (left panel). Quantitative analysis of the number of MOMA-2–positive macrophages infiltrated into the unligated (
n = 3–4 in each group) and ligated vessel (
n = 12–13 in each group) at day 3. *
P < 0.01 versus unligated WT mice;
†P < 0.05 versus unligated
Rock1+/– mice;
ΧP < 0.05 versus ligated WT mice.
