Mutation of β-glucosidase 2 causes glycolipid storage disease and impaired male fertility
J. Clin. Invest. Yildiz Yildiz, et al. 116:2985 doi:10.1172/JCI29224 [Go to this article.]

Figure 3
Sperm morphology in Gba2 wild-type and knockout mice. (A) Photomicrographs taken on light microscope showing normal sickle-headed sperm in wild-type mice and round-headed sperm in knockout mice. Original magnification, ×400. (B) Fluorescent staining for cell nuclei with DAPI (blue), immunofluorescent staining of acrosomes with peanut lectin (green), and fluorescent staining of mitochondria (red). Original magnification, ×630. (C) Electron microscopic analyses of sperm showing abnormal head shape, nuclear organization, and distribution of mitochondria in knockout sperm. Scale bars are indicated in individual panels.