SPDEF regulates goblet cell hyperplasia in the airway epithelium
J. Clin. Invest. Kwon-Sik Park, et al. 117:978 doi:10.1172/JCI29176 [
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Figure 3SPDEF and TTF-1 activate gene transcription in vitro. Reporter assays were performed using plasmids expressing SPDEF and TTF-1 and reporter plasmids in which
firefly luciferase gene (
luc) is controlled by
Sftpa (
A),
Foxj1 (
B),
Scgb1a1 (
C), and
Sox17 (
D) gene promoters as described in Methods. SPDEF activated
Sftpa,
Foxj1,
Scgb1a1, and
Sox17 promoters in the presence and absence of TTF-1. Controls (Con) were cells transfected with the reported and empty expression plasmids. Assays were repeated in triplicate in at least 3 experiments with similar results. Values are mean ± SD (
n = 3).
P values shown were obtained by ANOVA.
