(A) Maps of the Cadps2 gene, the targeting vector, and the resultant targeted allele are shown. Probes for Southern blot analysis to screen for targeted ES clones are indicated by small solid bars. Restriction enzyme sites include the following: B, BamHI; E1, EcoRI; E5, EcoRV; S, SmaI; X, XhoI. DTA, diphtheria toxin A fragment (B) Southern blot analysis, using the probes indicated in A, of DNA from tails of a single litter derived from a cross between heterozygotes. +/+, WT mice; +/–, heterozygous mice; –/–, homozygous mice. (C) RT-PCR banding patterns of CADPS2 using brain total RNA of P21 WT, heterozygous, and homozygous mice. The GAPDH gene was used as a constantly expressed control mRNA. (D) Immunoblot analysis of the cerebellum, neocortex, and hippocampus of P8 WT, heterozygous, and homozygous mice. Protein lysates from these brain regions were immunoblotted with anti-CADPS2 antibody. Cb, cerebellum; Cx, neocortex; Hip, hippocampus.