Endocarditis and biofilm-associated pili of Enterococcus faecalis
J. Clin. Invest. Sreedhar R. Nallapareddy, et al. 116:2799 doi:10.1172/JCI29021 [Go to this article.]

Figure 3
Ebp proteins form SrtC-dependent pili. (A) Pili on the surface of E. faecalis. Strain OG1RF grown in BHI was negatively stained with uranyl acetate and viewed by TEM. (B–G) Immunogold labeling and TEM of OG1RF pili stained with anti-Ebp antibodies. (H) Immunogold labeling and TEM of the ebpC disruption mutant stained with anti-EbpC antibodies. (I) Immunogold labeling and TEM of OG1RF stained with endocarditis patient serum. (J–L) Immunogold labeling and SEM of OG1RF pili stained with anti-Ebp antibodies. The anti-Ebp antisera used in each micrograph is indicated. The mixture of the 3 preimmune sera is shown as SEM control. Scale bars: 200 nm.