Endocarditis and biofilm-associated pili of Enterococcus faecalis
J. Clin. Invest. Sreedhar R. Nallapareddy, et al. 116:2799 doi:10.1172/JCI29021 [
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Figure 2Ebp proteins form an SrtC-dependent HMW ladder. (
A–
D) Immunoblots of mutanolysin extracts of wild-type
E. faecalis OG1RF;
ebpA disruption (TX5421),
ebpB disruption (TX5460),
ebpC disruption (TX5448), and
srtC deletion (TX5470) mutants; and
ebpC disruption mutants complemented with
ebpC (TX5476) or
ebpC plus
srtC (TX5479) probed with anti-Ebp antibodies. rec-, recombinant. (
E) OG1RF mutanolysin extracts, either left untreated or treated with periodate, were probed with anti-Ebp sera. (
F) Immunoblots of varying amounts of mutanolysin extracts (ME) of OG1RF grown in either TSBG or 40% horse serum and probed with anti-Ebp antibodies. The affinity-purified anti-Ebp Igs or anti-Ebp sera used in each Western blot are indicated. The
srtC deletion mutant sample in
A and the
ebpB disruption mutant sample in
B are from different gels.
