Interaction of mature CD3⁺CD4⁺ T cells with dendritic cells triggers the development of tertiary lymphoid structures in the thyroid
J. Clin. Invest. Tatjana Marinkovic, et al. 116:2622 doi:10.1172/JCI28993 [Go to this article.]

Figure 3
CD3⁺CD4⁺ T cells promote entry of B cells in the thyroid. (A) CD4⁺ and CD8⁺ T cells and B220⁺ B cells were sorted from the spleen of GFP-transgenic mice and injected separately into RAGTGCCL21 mice for 5 days. While CD3⁺CD4⁺GFP⁺ cells infiltrated the recipient thyroids in significant numbers, very few B220⁺ or CD3⁺CD8⁺ T cells were found (n = 4). In all cases, GFP⁺ cells were readily detected in lymph nodes. (B) GFP⁺CD3⁺CD4⁺ T cells (1 × 10⁶) were injected i.v. into RAGTGCCL21 mice. At day 10, mice were injected again with B220⁺ cells purified from C57Bl/6 mice. As a control, RAGTGCCL21 mice were injected with B220⁺ cells alone. Mice were sacrificed 24 hours later, and thyroids were examined for the presence of B cells. Shown are representative sections (n = 3 mice). Scale bars: 0.25 mm.