Interaction of mature CD3⁺CD4⁺ T cells with dendritic cells triggers the development of tertiary lymphoid structures in the thyroid
J. Clin. Invest. Tatjana Marinkovic, et al. 116:2622 doi:10.1172/JCI28993 [Go to this article.]

Figure 2
Kinetics of CCL21-driven entry of splenocytes (spl. into the thyroid. GFP⁺ splenocytes (10⁷) were injected into RAGTGCCL21 mice. At the indicated time points, thyroids and lymph nodes were analyzed for the presence of GFP⁺ cells. (A) Few GFP⁺ cells were found on days 1 and 3 (insets), but their number increased on day 5. Results shown are representative of 80 thyroid sections obtained from 4 mice at each time point. Magnification, ×40 (insets). (B) Splenocytes (10⁷) from GFP-transgenic mice deficient for CCR7 (GFP⁺/CCR7^–/–) were injected into RAGTGCCL21 mice. Ten days later, thyroids and lymph nodes of the recipient mice were extensively analyzed (more than 20 sections/thyroid) for the presence of GFP⁺ cells. Shown are representative sections (n = 3 mice). (C) All GFP⁺ cells present on days 1 and 3 in thyroids of RAGTGCCL21 mice stained with anti-CD3 antibody (n = 4 mice in each group). Most GFP⁺ cells on day 5 are CD3⁺; however, at this time point, few CD19⁺ B cells could also be detected (n = 4 mice). Scale bars: 0.05 mm.