Tumors induce a subset of inflammatory monocytes with immunosuppressive activity on CD8⁺ T cells
J. Clin. Invest. Giovanna Gallina, et al. 116:2777 doi:10.1172/JCI28828 [Go to this article.]

Figure 3
Evaluation of cytokines and cytokine receptors required by tumor-induced CD11b⁺ cells to inhibit generation of alloreactive and tumor-specific CTLs. CD11b⁺ sorted cells from tumor-bearing WT or different KO mice were added at a final concentration of 3% to a mixed leukocyte culture set up with BALB/c effectors stimulated with an equal number of C57BL/6 splenocytes. After 5 days, cytotoxic activity was tested in a 5-hour ⁵¹Cr-release assay against either a syngeneic control target (CT26, H-2^d) or an allogeneic target (MBL-2, H-2^b). Effector lymphocytes were taken from WT (A), IFN-γ KO (B), or IL-4 KO (C) mice. LU₃₀, defined as the number of lymphocytes necessary to achieve 30% specific lysis of 2 × 10³ target cells in a 5-hour assay, was calculated on the basis of the total number of viable cells recovered from the cultures. Data are expressed as the ratio between the LU₃₀ measured in cultures containing the third-party cells and in control cultures set up in the absence of third-party cells. Data are mean ± SEM from 3 experiments.