Proteasome-mediated degradation of IκBα and processing of p105 in Crohn disease and ulcerative colitis
J. Clin. Invest. Alexander Visekruna, et al. 116:3195 doi:10.1172/JCI28804 [Go to this article.]

Figure 5
Proteasomal processing of NF-κB precursor protein p105. (A) Kinetics of processing of p105 by proteasomes from the colonic mucosa of IBD patients and controls (CD, n = 6; UC, n = 5; controls, n = 5). ³⁵S-labeled in vitro–translated p105 (Supplemental Figure 1) was incubated with cytosolic lysates of colonic tissues in the presence of 10 mM ATP. A representative autoradiogram is shown for each group of patients. (B) Detailed analysis of processing of p105 during first 20 minutes by lysates from CD patients. (C) Processing of p105 in the absence of ATP by lysates from CD patients. (D) Quantitative analysis of autoradiograms, showing significant differences in the processing of p105 among CD patients, UC patients, and controls. Data represent mean values ± SEM. P < 0.0001 by 1-way ANOVA.