CD40Ig treatment results in allograft acceptance mediated by CD8⁺CD45RC^low T cells, IFN-γ, and indoleamine 2,3-dioxygenase
J. Clin. Invest. Carole Guillonneau, et al. 117:1096 doi:10.1172/JCI28801 [Go to this article.]

Figure 5
Grafts accepted long term after CD40Ig treatment showed strong accumulation of transcripts for regulatory molecules. Heart grafts from syngeneic donors or from allogeneic donors were harvested 120 days after transplantation. Recipients from allogeneic donors were treated with CD40Ig. (A) Heart total RNA was analyzed using real-time quantitative RT-PCR. Symbols indicate values for individual animals in arbitrary units. *P < 0.05, and **P < 0.01 versus syngeneic animals. (B) Confocal analysis of allogeneic or syngeneic (inserts) grafts using anti-CD8α (red) or anti–IFN-γ (green) antibodies. Merge analysis shows IFN-γ expression almost exclusively restricted to CD8⁺ cells (arrows), whereas some cells are IFN-γ^– (arrowheads). Identical results were obtained with 3 grafts from CD40Ig-treated animals and from adoptively transferred grafts. Original magnification, ×400.