CD40Ig treatment results in allograft acceptance mediated by CD8⁺CD45RC^low T cells, IFN-γ, and indoleamine 2,3-dioxygenase
J. Clin. Invest. Carole Guillonneau, et al. 117:1096 doi:10.1172/JCI28801 [Go to this article.]

Figure 4
Analysis of the TCR repertoire in CD8⁺CD45RC^low T cells. CD8⁺CD45RC^low or CD8⁺CD45RC^high T cells were purified from spleen of CD40Ig-treated or naive animals. A qualitative and quantitative analysis of the TCR Vβ transcriptome was performed as described in Methods. Each histogram represents a qualitative immunoscope analysis of the Vβ11 family repertoire of 1 animal. The histograms display the intensity of fluorescence in arbitrary units as a function of runoff CDR3 Vβ11 length in nucleotides. The CDR3 length distribution was unaltered (gaussian) in CD8⁺CD45RC^low from all naive animals or CD8⁺CD45RC^high from CD40Ig-treated recipients and altered (non-gaussian) in spleen CD8⁺CD45RC^low T cells from all CD40Ig-treated recipients with a predominant CDR3 of always the same length (27 nucleotides, arrows).