CD40Ig treatment results in allograft acceptance mediated by CD8⁺CD45RC^low T cells, IFN-γ, and indoleamine 2,3-dioxygenase
J. Clin. Invest. Carole Guillonneau, et al. 117:1096 doi:10.1172/JCI28801 [Go to this article.]

Figure 3
Expression of IFN-γ and regulatory molecules by CD8⁺CD45RC^low T cells. (A) Intracytoplasmic analysis of IFN-γ production in total splenocytes and in CD8⁺CD45RC^low and CD8⁺CD45RC^high cells from CD40Ig-treated or adoptively transferred animals. Cells were harvested, stimulated for 7 hours using phorbol myristate acetate and ionomycin, and analyzed with a FITC-labeled anti–IFN-γ mAb. Results are expressed as percent of positive cells. Each symbol indicates a value for an individual animals. Lines indicate mean ± SD. ATS, adoptively transferred. *P < 0.05, **P < 0.01, and ***P < 0.001. (B) Quantitative analysis of transcript accumulation was performed after isolation in CD8⁺CD45RC^low or CD8⁺CD45RC^high T cells isolated from CD40Ig-treated recipients or in CD8⁺CD45RC^low cells from naive animals. Results are expressed in arbitrary units of molecules normalized to HPRT ± SD. GITR, glucocorticoid-induced tumor necrosis factor receptor family–related gene. *P < 0.05, **P < 0.01, and ***P < 0.001.