CD40Ig treatment results in allograft acceptance mediated by CD8⁺CD45RC^low T cells, IFN-γ, and indoleamine 2,3-dioxygenase
J. Clin. Invest. Carole Guillonneau, et al. 117:1096 doi:10.1172/JCI28801 [Go to this article.]

Figure 2
CD8⁺CD45RC^low T cells mediate transfer of transplantation tolerance after CD40Ig treatment. (A) CD8⁺CD45RC^low and CD8⁺CD45RC^high T cells were sorted to greater than 99% purity from spleens. (B) Cells were purified from naive, CD40Ig-treated, or adoptively transferred recipients 120 days after transplantation. A total of 2.5 × 10⁶ cells of each cell population was injected i.v. the day of transplantation of LEW.1W hearts into naive LEW.1A recipients sublethally irradiated (4.5 Gy) at day –1. Graft survival was assessed by abdominal palpation of cardiac beating. Black line, CD8⁺CD45RC^low T cells from CD40Ig-treated animals (n = 4). Dotted line, CD8⁺CD45RC^low T cells from adoptively transferred animals (n = 2). Gray line, CD8⁺CD45RC^high T cells from CD40Ig-treated animals (n = 4). Dashed gray line, CD8⁺CD45RC^low T cells from naive animals (n = 4). P < 0.01 for CD8⁺CD45RC^low from CD40Ig-treated animals versus CD8⁺CD45RC^high and CD8⁺CD45RC^low from naive animals.