CD40 induces macrophage anti–Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes
J. Clin. Invest. Rosa M. Andrade, et al. 116:2366 doi:10.1172/JCI28796 [Go to this article.]

Figure 5
Activated CD4⁺ T cells induce vacuole/lysosome fusion through CD40/CD154 interaction. Peritoneal macrophages from WT mice (A) or IFN-γ^–/– mice (B) were infected with T. gondii–YFP and incubated for 10 minutes; this was followed by removal of extracellular parasites. After 1 hour, either resting (R) or activated (A) CD4⁺ T cells from WT (A) or IFN-γ^–/– (B) mice were added in the presence of anti-CD54 or control mAbs. Colocalization of LAMP-1 around PV was assessed by immunofluorescence 8 hours after infection. Results are shown as the mean ± SD and are representative of 4 independent experiments.