CD40 induces macrophage anti–Toxoplasma gondii
activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes
J. Clin. Invest. Rosa M. Andrade, et al. 116:2366 doi:10.1172/JCI28796 [
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Figure 3CD40 stimulation induces convergence of PV and late endosomes/lysosomes. Mouse resident peritoneal macrophages were incubated with control or stimulatory anti-CD40 mAbs, then challenged with
T. gondii–secRFP. Expression of LAMP-1 was assessed at 8 hours after challenge. Inset represents an extracellular tachyzoite with accumulation of fluorescence in dense granules. Macrophages were also incubated with heat-killed
T. gondii–secRFP, and LAMP-1 expression was assessed at 1 hour after challenge. Arrowheads indicate colocalization of LAMP-1 around
T. gondii–containing compartments. Scale bar: 5 μm. Results are representative of 4 independent experiments.
