Androgen-dependent pathology demonstrates myopathic contribution to the Kennedy disease phenotype in a mouse knock-in model
J. Clin. Invest. Zhigang Yu, et al. 116:2663 doi:10.1172/JCI28773 [
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Figure 4Testosterone-treated AR113Q females exhibit normal life span. (
A–
D) Testosterone-dependent effects on heterozygous AR113Q females. AR113Q females were implanted with testosterone (+T,
n = 8) or vehicle control (–T,
n = 7) pellets at 2–4 months. Serum testosterone levels, determined at 4–7 months, were significantly increased in hormone-treated mice compared with controls (
P < 0.001) (
A). Excretion of major urinary proteins (
B) was compared in AR113Q–T and AR113Q+T females. AR113Q+T females showed a small but significant deficit in forelimb grip strength (
C) (
P = 0.01 by unpaired Student’s
t test) but unchanged body mass (
D) (
P = 0.3 by unpaired Student’s
t test) compared with AR113Q–T females at 16 months. (
E) Survival curves of AR113Q+T (
n = 8) and AR113Q–T (
n = 7) (overlapping green lines), untreated AR113Q females (blue line,
n = 11), and WT females (red line,
n = 19) were similar (
P > 0.05). None of the AR113Q+T or AR113Q–T females died during the study; half were euthanized at 16–19 months, and the remainder were euthanized at 23–26 months.
