TNF-α downregulates eNOS expression and mitochondrial biogenesis in fat and muscle of obese rodents
J. Clin. Invest. Alessandra Valerio, et al. 116:2791 doi:10.1172/JCI28570 [Go to this article.]

Figure 1
eNOS expression is reduced in WAT from obese animals. (A) eNOS mRNA levels, measured by means of quantitative RT-PCR in WAT of genetically obese mice (ob/ob) and rats (fa/fa) and of environmentally obese mice (DIO) compared to respective controls (+/+, wild-type; SC, standard chow–fed animals). The cycle number at which the various transcripts were detectable was compared with that of β-actin as an internal control and expressed as arbitrary units versus values in control animals taken as 1.0 (n = 5 experiments). ***P < 0.01. (B) eNOS protein was detected by immunoblot analysis (1 experiment representative of 5 reproducible ones) in WAT of ob/ob mice, fa/fa rats, and DIO mice compared to respective controls. Numbers indicate the relative values from the densitometric analysis (normalized to β-actin) relative to controls, which were assigned a value of 1.0.