Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. 116:12 doi:10.1172/JCI28510 [Go to this article.]

Figure 4
Nuclear DNA content analysis of GFP⁺ adipocytes.

Adipocytes were isolated from GFP⁺ BMT mice fed control, ROSI-impregnated, or high-fat diets for 7 weeks. Adipocytes were permeablized with saponin and stained with PI. Flow cytometry was conducted with singlet discrimination. GFP-negative cells were excluded from the analysis. The position of polyploid (fused or multinuclear) cells or cells in the G₀/G₁ and G₂/M regions of the cell cycle histogram are indicated. The small peak to the left of the G₀/G₁ peak in each histogram indicates apoptotic cells. The average percentage of polyploid cells in each treatment is indicated in parentheses below the label for each treatment histogram.