Adipocytes were isolated from GFP+ BMT mice fed control, ROSI-impregnated, or high-fat diets for 7 weeks. Adipocytes were permeablized with saponin and stained with PI. Flow cytometry was conducted with singlet discrimination. GFP-negative cells were excluded from the analysis. The position of polyploid (fused or multinuclear) cells or cells in the G0/G1 and G2/M regions of the cell cycle histogram are indicated. The small peak to the left of the G0/G1 peak in each histogram indicates apoptotic cells. The average percentage of polyploid cells in each treatment is indicated in parentheses below the label for each treatment histogram.