Reduced maternal expression of adrenomedullin disrupts fertility, placentation, and fetal growth in mice
J. Clin. Invest. Manyu Li, et al. 116:2653 doi:10.1172/JCI28462 [
Go to this article.]
Figure 6Fetal
AM gene expression is upregulated in invasive TGCs.
(
A) Fluorescence microscopy of preimplanting blastocysts generated from an
AM+/– intercross and flushed from the uterine horn of an
AM+/– female at E3.5. Note the strong fluorescence present in both mural and polar trophectoderm cells and a fainter signal in the inner cell mass. The left blastocyst is
AM–/– and has approximately twice the intensity of fluorescence as the right
AM+/– blastocyst. (
B) Confirmation of TGC differentiation in a TS cell line using
Hand1 and
proliferin gene expression markers. Results from the quantitative RT-PCR experiment show the fold change in gene expression in differentiated TGCs compared with that in the undifferentiated TS cell culture, which was arbitrarily set at 1. (
C) Quantitative measurement of
AM gene expression in differentiated TGCs compared with that in the undifferentiated TS cell culture. All data were normalized to a GAPDH internal control. **
P < 0.001 compared with undifferentiated TS cells.
