Reduced maternal expression of adrenomedullin disrupts fertility, placentation, and fetal growth in mice
J. Clin. Invest. Manyu Li, et al. 116:2653 doi:10.1172/JCI28462 [Go to this article.]

Figure 3
Abnormal implantation spacing in AM^+/– females. (A) Timed natural matings or blastocyst transfers were performed in wild-type (white bars) and AM^+/– female mice (gray bars). The number of implanted embryos was determined between gestational stages E5.5 and E8.5. The number at the bottom of the bars represents the number of litters examined for each cross. (B) Evans blue dye injections of pregnant wild-type and AM^+/– female mice at gestational stages E4.5 and E5.5 revealed comparable conceptus sizes, demonstrating that timing of implantation was conserved in AM^+/– female mice. However, AM^+/– female mice displayed abnormal spacing of and overcrowded conceptuses in the uterine horn (arrowheads) compared with wild-type control females.