JCI - Heat shock protein 60 enhances CD4+ CD25+ regulatory T cell function via innate TLR2 signaling

Heat shock protein 60 enhances CD4⁺ CD25⁺ regulatory T cell function via innate TLR2 signaling

Alexandra Zanin-Zhorov, Liora Cahalon, Guy Tal, Raanan Margalit, Ofer Lider, Irun R. Cohen

Published in Volume 116, Issue 7

J Clin Invest. 2006; 116(7):2022–2032 doi:10.1172/JCI28423

Abstract | Full text | PDF

Options: View larger image (or click on image)

Figure 2

The CD4⁺ CD25⁺ population mediates the inhibitory effect of HSP60 on IFN-γ and TNF-α secretion in T cells.

(A) Unseparated CD4⁺ and CD4⁺CD25⁺-depleted T cells were incubated with HSP60 (1 ng/ml) for 2 hours, washed, and transferred to 24-well plates coated with anti-CD3 mAb (OKT; 0.5 μg/ml) in serum-free medium. The supernatants were analyzed for IFN-γ and TNF-α. (B) Unseparated CD4⁺, CD4⁺CD25⁺, or CD4⁺CD25^– T cells were checked for anti-CTLA4 and Foxp3 expression. One representative experiment of 5 is shown. (C–E) CD4⁺CD25⁺ T cells were incubated with HSP60 (1 ng/ml) for 2 hours, washed, mixed in the indicated proportions with CD4⁺CD25^– T cells (C) or with CD8⁺ T cells (E), and transferred to 24-well plates coated with anti-CD3 mAb (OKT; 0.5 μg/ml) in serum-free medium. The supernatants were analyzed for IFN-γ and TNF-α (C and E). Cell proliferation was determined after 96 hours at a concentration of 10% of CD4⁺CD25⁺ T cells (D). The means ± SD of 6 different donors are shown. *P < 0.05. t-ERK, total ERK.