The neurofibromin GAP-related domain rescues endothelial but not neural crest development in Nf1^–/– mice
J. Clin. Invest. 116:9 doi:10.1172/JCI28341 [Go to this article.]

Figure 3
HA-GRD expression rescues embryonic cardiovascular defects of Nf1^–/–.

Representative cross-sections (magnification, ×4) of hearts through the developing endocardial cushions (dashed line) with high-power insets (magnification, ×20) of left ventricular wall from E12.5 wild-type (A); Nf1^–/–, HA-GRD^k/+ (B); and Nf1^–/–, HA-GRD^k/+, CMV-cre⁺ (C) littermates. Right ventricles (rv) and left ventricles (lv) are indicated. Arrowheads in the insets highlight thickness of the ventricular wall. The developing endocardial cushions of B were enlarged compared with those in A. In addition, there was a large pericardial effusion (asterisk in B), suggestive of cardiovascular failure, and a thinned ventricular myocardium (inset). Expression of HA-GRD mediated by a CMV-cre transgene (C) rescued these cardiovascular defects.