PSGL-1–mediated activation of EphB4 increases the proangiogenic potential of endothelial progenitor cells
J. Clin. Invest. Philippe Foubert, et al. 117:1527 doi:10.1172/JCI28338 [Go to this article.]

Figure 9
E selectin and P selectin mediate ephrin-B2–Fc–induced EPC adhesion. (A) Ephrin-B2–Fc treatment increased EPC adhesion to immobilized E selectin and P selectin fusion proteins. EPCs were stimulated with 3 μg/ml of either ephrin-B2–Fc, EphB4-Fc, or CD6-Fc. Then the cells were allowed to adhere to immobilized recombinant E selectin–Fc (black bars), P selectin–Fc (gray bars), or to human IgG Fcγ fragments (white bars). Adhesion was quantified by measuring OD at 570 nm. Results are expressed as percentages of control nonstimulated EPCs (EPC). EPCs represented in this figure were nonstimulated. n = 3. *P < 0.05; **P < 0.01 versus nonstimulated EPCs. (B) Blocking antibodies directed against E selectin and P selectin neutralized ephrin-B2–Fc–induced EPC adhesion to IL-1β–prestimulated HUVEC monolayers. n = 3. *P < 0.05 versus EPCs treated with control IgG1.