PSGL-1–mediated activation of EphB4 increases the proangiogenic potential of endothelial progenitor cells
J. Clin. Invest. Philippe Foubert, et al. 117:1527 doi:10.1172/JCI28338 [
Go to this article.]
Figure 7EphB4 activation mediates EPC adhesion through PSGL-1. (
A and
B) PSGL-1 expression in EPCs. EPCs were stimulated with 3 μg/ml of either ephrin-B2–Fc, EphB4-Fc, or CD6-Fc and then processed for FACS analysis of PSGL-1 expression. (
A) FACS profiles of nontransfected (top panel) and EphB4 siRNA–transfected (bottom panel) EPCs. (
B) Expression of PSGL-1 in nontransfected (top panel) and EphB4 siRNA–transfected (bottom panel) EPCs.
n = 3. **
P < 0.01 versus nonstimulated EPCs. (
C) Effect of PSGL-1 siRNA on PSGL-1 protein expression. Control siRNA– and PSGL-1 siRNA–transfected EPCs were stimulated with 3 μg/ml ephrin-B2–Fc or left unstimulated and then processed for immunocytochemistry with an anti–PSGL-1 antibody as described in Methods. PSGL-1–positive staining appears in red. Scale bar: 20 μm. (
D) Effect of PSGL-1 siRNA on EPC adhesion to IL-1β prestimulated HUVEC monolayer. Adhesion was quantified by measuring OD at 570 nm.
n = 3. *
P < 0.05 versus nonstimulated EPCs transfected with control siRNA.
