JCI - A public T cell clonotype within a heterogeneous autoreactive repertoire is dominant in driving EAE

A public T cell clonotype within a heterogeneous autoreactive repertoire is dominant in driving EAE

Juscilene S. Menezes, Peter van den Elzen, Jordan Thornes, Donald Huffman, Nathalie M. Droin, Emanual Maverakis, Eli E. Sercarz

Published in Volume 117, Issue 8

J Clin Invest. 2007; 117(8):2176–2185 doi:10.1172/JCI28277

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Figure 4

CD4⁺ BV8S2/BJ2S7-DAGGGY clonotypic T cells induce EAE.

(A) Cells from mice immunized with Ac1–9 8 days earlier were stimulated in vitro with the peptide and IL-12; sorted into CD4+, CD4+BV8.2+, and CD4+BV8.2– populations; and adoptively transferred into naive mice. (B) The BV8.2⁺ T cell clone bearing the DAGGGY motif in the CDR3 region and the Ac1–9–specific T cell line depleted of BV8.2⁺ T cells were stimulated in vitro with the peptide and IL-12, washed, and adoptively transferred into naive mice. The numerical range of cells transferred was adjusted for different populations as described in Methods. Mice were monitored daily for clinical signs of EAE.