Constraints in antigen presentation severely restrict T cell recognition of the allogeneic fetus
J. Clin. Invest. Adrian Erlebacher, et al. 117:1399 doi:10.1172/JCI28214 [
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Figure 7Defective OT-I cell priming in pregnant mice bearing Act-mOVA concepti. (
A) Impaired cell accumulation. The number of OT-I cells remaining after transfer into Act-mOVA–mated females was calculated as a percentage of total CD8
+ T cells, then normalized to the number of cells remaining after equivalent time periods in B6-mated mice injected in parallel. Similarly, the number of OT-I cells remaining in virgin females treated with OVA or OVA plus anti-CD40 antibodies and poly(I:C) was normalized to the number remaining in untreated virgins. Each point represents mean ± SEM of
n = 3–10 Act-mOVA–mated or
n = 2–3 virgin mice. (
B) Impaired modulation of CD62L and CD25 expression. OT-I cells were analyzed either 7 days after transfer into E10.5 Act-mOVA–mated or in virgin mice 3 days after treatment with OVA plus anti-CD40 antibodies and poly(I:C). Plots are representative of 3 independent experiments, each with individual mice or pools of
n = 2–5 Act-mOVA–mated and
n = 1–3 virgin mice per experiment. Plots display similar cell numbers in the 2 groups of mice, even though this does not represent their relative prevalence in vivo. The percentage of OT-I cells with altered marker expression is shown. (
C and
D) Impaired cytokine expression. Seven days after transfer into E10.5 pregnant mice, OT-I cells in the spleen (
C) or uterine LNs (
D) were stained for intracellular cytokines. Virgin females were similarly analyzed 7 days after indicated treatments given at the time of OT-I transfer. The percentage of OT-I cells with a CFSE
locytokine
+ phenotype is shown. Data are representative of 2 independent experiments, each with a pool of
n = 4 Act-mOVA–mated mice. The other treatment groups show data representative of individual mice or pools encompassing a total of
n = 2–6 mice per group.
