Constraints in antigen presentation severely restrict T cell recognition of the allogeneic fetus
J. Clin. Invest. Adrian Erlebacher, et al. 117:1399 doi:10.1172/JCI28214 [Go to this article.]

Figure 4
Indirect presentation of fetal/placental OVA to maternal OT-I T cells. (A) OT-I cells on a B6CBAF1 background are able to detect K^b/SIINFEKL complexes in (bm1 × CBA)F₁ mice. Splenocytes from (bm1 × CBA)F₁ or B6CBAF1 female virgins were analyzed 2 days after the injection of CFSE-labeled B6CBAF1 OT-I cells, with or without a concurrent injection of SIINFEKL-loaded B6CBAF1 splenocytes. Histograms were normalized to account for the loss of OT-I cells in (bm1 × CBA)F₁ mice. (B) Lack of OT-I proliferation in (bm1 × CBA)F₁ females mated to Act-mOVA males. Splenocytes from B6- or Act-mOVA–mated (bm1 × CBA)F₁ females were analyzed 2 days after the injection of CFSE-labeled B6CBAF1 OT-I cells on or after E12.5. Data are representative of n = 10 Act-mOVA–mated (bm1 × CBA)F₁ and n = 3 B6-mated mice in 3 independent experiments. Identical results were obtained with LN cells.