Constraints in antigen presentation severely restrict T cell recognition of the allogeneic fetus
J. Clin. Invest. Adrian Erlebacher, et al. 117:1399 doi:10.1172/JCI28214 [Go to this article.]

Figure 2
OVA shed from the conceptus accumulates on FDCs. (A–G) OVA accumulation on splenic FDCs. (A–D) E17.5 spleens from Act-mOVA–mated (A and B) or B6-mated (C and D) mice double stained for OVA (red; A and C) and CD35 (green; B and D). Red pulp is apparent as areas of diffuse green staining resulting from rbc rendered artifactually fluorescent by the immunohistochemistry protocol. Data are representative of n = 8–9 mice per group on E15.5–E17.5. Scale bar: 0.5 mm. (E–G) High-power confocal photomicrographs of a double-stained OVA⁺ focus, showing OVA staining (E), CD35 staining (F), and the merged image (G). (H–J) OVA accumulation on LN FDCs. (H) Two subcutaneous LNs from an Act-mOVA–mated mouse on E17.5; a higher magnification of the OVA⁺ focus indicated with an arrow is shown in I. Data are representative of all n = 8 mice analyzed at E15.5–E17.5. (J) An OVA⁺ focus in a uterine LN on E11.5, as such foci begin to appear. Similar foci were seen in n = 3 of 4 mice analyzed. Scale bar: 1 mm (H) and 0.2 mm in (I and J). As in C, LNs from B6-mated mice showed no OVA⁺ foci (data not shown).