Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells
J. Clin. Invest. Jeffrey J. Ross, et al. 116:3139 doi:10.1172/JCI28184 [Go to this article.]

Figure 6
Characterization of L-type Ca²⁺ channels by whole-cell patch clamp in F-MAPC-SMCs. (A) A representative current recording trace of a day 0 F-MAPC or a passage 1 F-MAPC-SMC differentiated with TGF-β1 plus PDGF-BB. The inward calcium current induced by 300 nM FPL64176 (L-type calcium channel opener) in passage 1 F-MAPC-SMCs was completely inhibited by 3 μM nifedipine (right), while current was not induced to a significant extent by 300 nM FPL64176 (left). (B) Corresponding I-V plot for F-MAPC and passage 1 F-MAPC-SMC differentiated with TGF-β1 plus PDGF-BB. Current is given in pA/pF and membrane potential in mV. Cells were voltage clamped at a holding potential of –90 mV, and currents were evoked by +10-mV steps to +50 mV using test pulses of 120-ms duration. (C) Quantification of inward current in the presence of FPL64176 while the membrane potential was held at 0 mV for each cell group demonstrates the development of significant inward current in day 6 TGF-β1 plus PDGF-BB–differentiated F-MAPC-SMCs and inward current similar to that in RAOSMCs by passage 1 in both TGF-β1– and TGF-β1 plus PDGF-BB–differentiated F-MAPC-SMCs (*P < 0.05, **P < 0.01 compared with F-MAPCs; n = 8–10).