Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells
J. Clin. Invest. Jeffrey J. Ross, et al. 116:3139 doi:10.1172/JCI28184 [
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Figure 3SMC-specific proteins are expressed in SD-MAPCs differentiated with TGF-β1. (
A) Temporal characterization of SM22α and α-SMA expression on days 0, 2, 4, and 6 (magnification, ×40). SM22α was detected at low levels on day 0, which increased and localized to stress fibers as early as day 2. α-SMA expression was absent on day 0 and detected by day 4. (
B) Colocalization of SM22α (green) and α-SMA (red) further demonstrates that expression of SM22α precedes that of α-SMA. (
C) IgG control. (
D) Expression of the SMC-specific proteins calponin, α-SMA, SM-MHC, and SM22α in SD-MAPC-SMCs passaged once in expansion medium. Representative example of more than 5 experiments; insets: magnificiation, ×10.
