Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells
J. Clin. Invest. Jeffrey J. Ross, et al. 116:3139 doi:10.1172/JCI28184 [
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Figure 1Effects of TGF-β1, PDGF-BB, or TGF-β1 plus PDGF-BB on SD-MAPC morphology. Phase-contrast images of SD-MAPCs (
A); SD-MAPCs treated for 6 days in serum-free medium with 5 ng/ml PDGF-BB (
B), 2.5 ng/ml TGF-β1 (
C), or 5 ng/ml PDGF-BB and 2.5 ng/ml TGF-β1 (
D); or primary RAOSMCs (
E). Morphology is representative of more than 10 independent experiments. Magnification, ×10. Phalloidin staining (
A–
E, insets; magnification, ×10) demonstrates that the stress fiber pattern of SD-MAPCs induced with TGF-β1 or TGF-β1 plus PDGF-BB is similar to that of primary RAOSMCs (
E). (
F) Cellular density on day 6 of SD-MAPC differentiation (
P < 0.01;
n = 4).
