(A) Characterization of BMT. C57BL/6 mice were subjected to lethal irradiation followed by BMT by receiving bone marrow cells from WT (WT to WT), Bmx-KO mice (KO to WT), or Bmx-SK-Tg mice (Tg to WT). Success of BMT was controlled by genotyping of Bmx gene 6 weeks after BMT. The mice were then subjected to femoral artery ligation as described in Methods. Four weeks after ischemia, bone marrow was harvested, and expression of Bmx and Bmx-SK in BM was determined by Western blotting with anti-Bmx antibody. Bone marrow from WT and Bmx-SK-Tg mice without BMT/ligation was used as control. Expression of Bmx and Bmx-SK is indicated. Anti–β-tubulin antibody was used for normalization. (B) Pedal blood flow recovery after femoral artery ligation in C57BL/6 (WT Ctrl) versus mice that received bone marrow from WT (BMT of WT to WT), Bmx-KO mice (BMT of KO to WT), or Bmx-SK-Tg mice (BMT of Tg to WT). BMT was performed, and pedal blood flow recovery on indicated days after femoral artery ligation was assessed by laser Doppler imaging. *P < 0.05. (C) BMT of WT to Bmx-KO mice. Bmx-KO mice were subjected to lethal irradiation followed by BMT by receiving bone marrow cells from WT mice (WT to KO). Successful BMT was controlled by genotyping of the Bmx gene 6 weeks after BMT. The mice were then subjected to femoral artery ligation as described in Methods. WT and Bmx-KO mice without BMT were used as controls. Pedal blood flow recovery after femoral artery ligation was assessed by laser Doppler imaging. n = 10 for each strain. *P < 0.05.