Macrophages and neutrophils are the targets for immune suppression by glucocorticoids in contact allergy
J. Clin. Invest. 117:5 doi:10.1172/JCI28034 [Go to this article.]

Figure 3
GC-mediated suppression of CHS requires GR actions in myeloid cells.

(A) Swelling response of GR^flox and GR^K14CreERT2 mice (shown as % increase in thickness; *P < 0.01; n = 5–6). All mice were sensitized at day 0 with vehicle (Veh) or DNFB and challenged with DNFB in the presence or absence of dexamethasone. (B) Swelling response of GR^flox and GR^lckCre mice treated as described in A. (C) FACS analysis of BM cells from GR^LysMCre mice crossed with RA/EG mice containing a tk-loxP-EGFP reporter gene (GR^LysMCre;RA/EG mice) to determine the expression of EGFP and GR-1. Numbers indicate the percentage of cells in each quadrant. (D) PCR analysis of genomic DNA isolated from GR-1⁺ granulocytes positive or negative for EGFP from GR^LysMCre;RA/EG mice in comparison with genomic DNA derived from GR^flox/GR^null heterozygous mice (50% per allele). (E) Swelling response of GR^flox and GR^LysMCre mice treated as described in A.